UBE2N Antibody

• Catalog Number: orb1243635
• Category: Antibodies
• Description: UBE2N Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Canine, Drosophila, Human, Mouse, Rat, Zebrafish
• Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human UBE2N.
• Concentration: batch dependent
• Dilution range: UBE2N antibody can be used for detection of UBE2N by ELISA at 1:1562500. UBE2N antibody can be used for detection of UBE2N by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 17 kDa
• Target: UBE2N
• UniProt ID: P61088
• NCBI: NP_003339
• Storage: For short periods of storage (days) store at 4°C. For longer periods of storage, store UBE2N antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
• Buffer/Preservatives: Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Alternative names: UBE2N, MGC131857, MGC8489, UBC13, UbcH-ben, UbcH13
• Note: For research use only
• Application notes: UBE2N antibody can be used for detection of UBE2N by ELISA at 1:1562500. UBE2N antibody can be used for detection of UBE2N by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
• Expiration Date: 12 months from date of receipt.

Antibody used in WB on Human Jurkat cells at 2.5 ug/ml.

Antibody used in IHC on Mouse brainstem at 1:500.

Antibody used in WB on Human NT-2, mouse brain at 2 ug/ml.

Antibody used in WB on r-UBE2N at 1:500 (Lanes: 1: 40 ng HIS-UBE2D1 protein, 2: 40 ng HIS-UBE2D2 protein, 3: 40 ng HIS-UBE2D3 proteinm, 4: 40 ng HIS-UBE2D4 protein, 5: 40 ng HIS-UBE2E1 protein, 6: 40 ng HIS-UBE2E2 protein, 7: 40 ng HIS-UBE2E3 protein, 8: 40 ng HIS-UBE2K protein, 9: 40 ng HIS-UBE2L3 protein, 10: 40 ng HIS-UBE2N protein, 11: 40 ng HIS-UBE2V1 protein, 12: 40 ng HIS-UBE2V2 protein.).