Trk B (phospho Tyr706) rabbit pAb

SKU: orb769305

Description

Rabbit polyclonal antibody to Trk B (phospho-Tyr706)

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Trk B around the phosphorylation site of Tyr705. AA range:671-720
• Molecular Weight: 92
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-NTRK2 antibody, anti-TRKB antibody, anti-BDNF/NT-3 growth factors receptor antibody, anti-GP145-TrkB antibody, anti-Trk-B antibody, anti-Neurotrophic tyrosine kinase receptor type 2 antibody, anti-TrkB tyrosine kinase antibody, anti-Tropomyosin-related kinase B antibody

Immunofluorescence analysis of Hela cell. 1, Trk B (phospho Tyr706) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Western Blot analysis of 293 cells using Phospho-Trk B (Y706) Polyclonal Antibody diluted at 1:1000.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Trk B (Phospho-Tyr705) Antibody.

Immunofluorescence analysis of HUVEC cells, using Trk B (Phospho-Tyr705) Antibody. The picture on the right is blocked with the phospho peptide.