TOLLIP Antibody (C-term)

• Catalog Number: orb1931670
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This TOLLIP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 221-250 amino acids from the C-terminal region of human TOLLIP.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• UniProt ID: Q9H0E2
• MW: 30282 Da
• Tested applications: IHC-P, WB
• Dilution range: WB: 1:1000, IHC-P: 1:50~100, IHC-P: 1:50~100
• Antibody Type: Primary Antibody
• Clone Number: RB4409
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Toll-interacting protein, TOLLIP
• Research Area: Cell Biology, Immunology & Inflammation
• Note: For research use only
• NCBI: NP_061882.2
• Expiration Date: 12 months from date of receipt.

Western blot analysis of TOLLIP (arrow) using rabbit polyclonal TOLLIP Antibody (A236). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the TOLLIP gene.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Middle panels, TOLLIP expression in matched normal and tumor breast samples. Here the TOLLIP stain in the invasive ductal carcinoma cells shows a much stronger signal compared with the normal ductal cells. Bottom panels, TOLLIP expression in matched normal and tumor breast samples. In this case, the signal for TOLLIP in the normal ductal cells is stronger than that in the invasive ductal carcinoma cells.