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| Catalog Number | orb766490 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to TLE4 |
| Clonality | Polyclonal |
| Tested applications | ELISA, IHC-P, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human TLE4. AA range:161-210 |
| Concentration | 1 mg/ml |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
| Conjugation | Unconjugated |
| MW | 83 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Alternative names | Anti-TLE4 antibody, anti-KIAA1261 antibody, anti-T Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western Blot analysis of various cells using TLE4 Polyclonal Antibody. Secondary antibody was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.
Western blot analysis of lysates from Jurkat, 293, and HUVEC cells, using TLE4 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4°C overnight.3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
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