Thymidine Kinase (phospho Ser13) rabbit pAb

• Catalog Number: orb770271
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Thymidine Kinase (phospho-Ser13)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Immunogen: The antiserum was produced against synthesized peptide derived from human TK around the phosphorylation site of Ser13. AA range:1-50
• MW: 25
• Tested applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-TK1 antibody, anti-Thymidine kinase antibody,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using TK (Phospho-Ser13) Antibody.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using TK (Phospho-Ser13) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HeLa cells treated with paclitaxel 1uM 24h, using TK (Phospho-Ser13) Antibody. The lane on the right is blocked with the phospho peptide.