Stat5a (phospho Ser780) rabbit pAb

• Catalog Number: orb770174
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Stat5a (phospho-Ser780)
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Immunogen: The antiserum was produced against synthesized peptide derived from human STAT5A around the phosphorylation site of Ser780. AA range:745-794
• MW: 91
• Tested applications: ELISA, IHC-P, IP, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/10000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-STAT5A antibody, anti-STAT5 antibody, anti-Si
• Note: For research use only

Western blot analysis of Stat5a (phospho Ser780) Polyclonal Antibody, using Hela, Jurkat cell treated or untreated with PMA 125ng/ml 30', 4°C over night, secondary antibody was diluted at 1:10000, 37°C 1hour.

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using STAT5A (Phospho-Ser780) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from HeLa cells, using STAT5A (Phospho-Ser780) Antibody. The lane on the right is blocked with the phospho peptide.