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| Catalog Number | orb770174 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to Stat5a (phospho-Ser780) |
| Clonality | Polyclonal |
| Tested applications | ELISA, IHC-P, IP, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human STAT5A around the phosphorylation site of Ser780. AA range:745-794 |
| Concentration | 1 mg/ml |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/10000. Not yet tested in other applications. |
| Conjugation | Unconjugated |
| MW | 91 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Alternative names | Anti-STAT5A antibody, anti-STAT5 antibody, anti-Si Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of Stat5a (phospho Ser780) Polyclonal Antibody, using Hela, Jurkat cell treated or untreated with PMA 125ng/ml 30', 4°C over night, secondary antibody was diluted at 1:10000, 37°C 1hour.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using STAT5A (Phospho-Ser780) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of lysates from HeLa cells, using STAT5A (Phospho-Ser780) Antibody. The lane on the right is blocked with the phospho peptide.
Phospho-STAT5a (Ser780) Rabbit pAb [orb2715699]
IHC-P, WB
Human, Mouse, Rat
Polyclonal
Unconjugated
100 μl, 50 μl
