SSBP1 Antibody

• Catalog Number: orb1256680
• Category: Antibodies
• Description: SSBP1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-148 of human SSBP1 (NP_001243442.1).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 14kDa
• Target: SSBP1
• UniProt ID: Q04837
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: Mt-SSB, SOSS-B1, SSBP, mtSSB, single-stranded DNA-
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:100IP: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using SSBP1 antibody (orb1256680) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry of paraffin-embedded human colon carcinoma using SSBP1 antibody (orb1256680) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human breast cancer using SSBP1 antibody (orb1256680) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse spinal cord using SSBP1 antibody (orb1256680) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse brain using SSBP1 antibody (orb1256680) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using SSBP1 antibody (orb1256680). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of MCF7 cells using 1 ug SSBP1 antibody (orb1256680). Western blot was performed from the immunoprecipitate using SSBP1 antibody (orb1256680) at a dilution of 1:1000.