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Catalog Number | orb1274608 |
---|---|
Category | Antibodies |
Description | SARS-CoV-2 (COVID-19) Nucleocapsid Antibody [75G5a] |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | 75G5a |
Tested applications | ELISA |
Reactivity | Virus |
Isotype | IgG1k |
Immunogen | SARS-CoV-2 nucleoprotein (NP) specific peptide |
Concentration | batch dependent |
Dilution range | Paired with 84C4a or 84D7, used for specific, high-sensitivity detection of SARS-CoV-2 nucleocapsid protein (NP). |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | N |
NCBI | QHD43423 |
Storage | SARS-CoV-2°COVID-19) Nucleoprotein Antibody [75G5a] can be stored at 4˚°C for three months and -20˚°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | 1X PBS, 0.02% NaN3, 50% Glycerol, 0.1% rAlbumin |
Alternative names | SARS-CoV-2 (COVID-19) Nucleoprotein Antibody [P001 Read more... |
Note | For research use only |
Application notes | Paired with 84C4a or 84D7, used for specific, high-sensitivity detection of SARS-CoV-2 nucleocapsid protein (NP). |
Expiration Date | 12 months from date of receipt. |
Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Nucleocapsid in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody (orb1274608, 1 ng/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 Nucleocapsid protein was observed in the macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.
Microtiter wells were coated with SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) and SARS-CoV NP at 1 ug/mL. Purified rabbit monoclonal antibody was serially diluted 1:2 starting at 1 ug/mL, and shows very strong and specific binding to COVID-19 NP antigen, with no significant cross-reactivity to SARS-CoV NP antigen.
A sandwich ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) specific rabbit monoclonal antibodies. COVID-19 NP was serially diluted 1:2 starting at 20 ng/mL.
A sandwich ELISA was performed using SARS-CoV-2 (COVID-19) Nucleocapsid Protein (NP) specific rabbit monoclonal antibodies. COVID-19 NP was serially diluted 1:2 starting at 25 ng/mL.
Western blot analysis of SARS-CoV2 and SARS-CoV nucleocapsid protein (50 ng) probed with 1 ug/mL CoV2NP rabbit monoclonal Antibody.