Saposin rabbit pAb

SKU: orb769720

Description

Rabbit polyclonal antibody to Saposin

Images & Validation

• Tested Applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human PSAP. AA range:307-356
• Molecular Weight: 58
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-PSAP antibody, anti-GLBA antibody, anti-SAP1 antibody, anti-Proactivator polypeptide antibody

Western Blot analysis of 3T3 cells using Saposin Polyclonal Antibody.

Western blot analysis of lysates from NIH/3T3 and RAW264.7 cells, using PSAP Antibody. The lane on the right is blocked with the synthesized peptide.

Western blot analysis of the lysates from HeLa cells using PSAP antibody.

Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).