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| Catalog Number | orb770748 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to RIP2 (phospho-Ser176) |
| Clonality | Polyclonal |
| Tested applications | ELISA, IHC-P, WB |
| Reactivity | Human, Mouse |
| Isotype | IgG |
| Immunogen | The antiserum was produced against synthesized peptide derived from human RIPK2 around the phosphorylation site of Ser176. AA range:146-195 |
| Concentration | 1 mg/ml |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications. |
| Conjugation | Unconjugated |
| MW | 70 |
| Source | Rabbit |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
| Alternative names | Anti-RIPK2 antibody, anti-CARDIAK antibody, anti-R Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western Blot analysis of cell lysis treated or untreated by LPS 100ng/mL 30min, using primary antibody at 1:1000 dilution. Secondary antibody was diluted at 1:10000.
Western Blot analysis of 293 cells using Phospho-RIP2 (S176) Polyclonal Antibody.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using RIPK2 (Phospho-Ser176) Antibody.
Immunohistochemistry analysis of paraffin-embedded human brain, using RIPK2 (Phospho-Ser176) Antibody. The picture on the right is blocked with the phospho peptide.
