Ref-1 rabbit pAb

• Catalog Number: orb766212
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Ref-1
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Immunogen: The antiserum was produced against synthesized peptide derived from human APEX1. AA range:191-240
• MW: 34
• Tested applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-APEX1 antibody, anti-APE antibody, anti-APE1
• Note: For research use only

Western Blot analysis of various cells using Ref-1 Polyclonal Antibody diluted at 1:2000.

Western Blot analysis of HeLa cells using Ref-1 Polyclonal Antibody diluted at 1:2000.

Western blot analysis of lysates from COLO205 and HeLa cells, using APEX1 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Tris-EDTA, pH9.0 was used for antigen retrieval. 2 Antibody was diluted at 1:200 (4°C overnight.3, Secondary antibody was diluted at 1:200 (room temperature, 45min).