Human CD73

SKU: orb2993538

Description

Recombinant Human 5'-Nucleotidase is produced by our Mammalian expression system and the target gene encoding Trp27-Lys547 is expressed with a 6His tag at the C-terminus.

Key Properties

• Expression System: Human Cells
• Biological Origin: Human
• Biological Activity: ELISA: Immobilized Human CD73-His(Cat#orb2993538) at 2μg/ml (100 μl/well) can bind Anti-Human CD73 mAb(Cat#orb2993120).The ED50 of Anti-Human CD73 mAb(Cat#orb2993120) is 3.33 ng/ml.. BLI: Loaded Anti-Human CD73 mAb-Fc(Cat#orb2993120) on Protein A Biosensor, can bind Human CD73-His(Cat#orb2993538) with an affinity constant of -3 nM as determined in BLI assay.
• Tag: C-6His
• Molecular Weight: Predicted: 58.76 KDa. Observed: 62 KDa, reducing conditions
• Expression Region: Trp27-Lys547
• Purity: SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxins: < 1 EU/µg as determined by LAL test.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
• Form/Appearance: Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 120mM NaCl, 4mM CaCl2, 20% Glycerol, pH 7.5.
• Disclaimer: For research use only

Alternative Names

5'-Nucleotidase; 5'-NT; Ecto-5'-Nucleotidase; CD73; NT5E; NT5; NTE

Quick Database Links

UniProt

AAH65937.1