Rad54 rabbit pAb

• Catalog Number: orb770630
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Rad54
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human RAD54L. AA range:221-270
• Concentration: 1 mg/ml
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
• Conjugation: Unconjugated
• MW: 85
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-RAD54L antibody, anti-RAD54A antibody, anti-D
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysates from HeLa cells, Jurkat cells, and 293 cells, using RAD54 Antibody. The lane on the right is blocked with the synthesized peptide.

Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).