You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1527531 |
---|---|
Category | Antibodies |
Description | ZBTB40 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human |
Immunogen | Between 1189 and C-terminus |
Antibody Type | Primary Antibody |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | ZBTB40 |
UniProt ID | Q9NUA8 |
NCBI | NP_055685.3 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | zinc finger and BTB domain-containing protein 40; Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human ZBTB40 by western blot and immunoprecipitation. Samples: Whole cell lysate from HEK293T (5, 15 and 50 µg for WB) and HeLa (1 mg for IP, 20% of IP loaded) cells. Antibodies: Affinity purified rabbit anti-ZBTB40 antibody orb1527531 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. ZBTB40 was also immunoprecipitated by rabbit anti-ZBTB40 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).
Detection of human ZBTB40 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-ZBTB40 (Cat. No. orb1527531) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB