Rabbit anti-VISTA Recombinant Monoclonal Antibody

SKU: orb1519912

Description

VISTA Antibody

Images & Validation

• Tested Applications: FC, ICC, IHC, IP, WB
• Dilution range: WB - 1:1000; IP - 20 ul/1mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; IHC-IF - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Clone No.: BLR035F
• Immunogen: Between 261 and C-terminus
• Target: VISTA
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 100 µg/ml
• Disclaimer: For research use only

Alternative Names

PP2135; V-set immunoregulatory receptor; V-set domain-containing immunoregulatory receptor; VISTA; sisp-1; stress-induced secreted protein-1; V-type immunoglobulin domain-containing suppressor of T-cell activation; SISP1; B7-H5; platelet receptor GI24; B7H5; C10orf54; DD1alpha; Death Domain1alpha; GI24; PD-1H; PDCD1 homolog; V-domain Ig suppressor of T cell activation

Detection of human VISTA (red) by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-VISTA recombinant monoclonal antibody (orb1519912) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).

Quick Database Links

Gene Symbol

VISTA

UniProt

Q9H7M9

RefSeq (Protein):NP_071436.1