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Rabbit anti-VE-Cadherin Recombinant Monoclonal Antibody
Description
Images & Validation
−| Tested Applications | ICC, IHC, IP, WB |
|---|---|
| Dilution range | WB - 1:1,000; IP - 12 µl/1 mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections. |
| Reactivity | Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Clone No. | BLR239K |
| Immunogen | Residues 25-595 (ECD) |
| Target | VE-Cadherin |
Storage & Handling
−| Storage | 2 - 8°C |
|---|---|
| Form/Appearance | Whole IgG |
| Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 500 µg/ml |
| Disclaimer | For research use only |
Alternative Names
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Detection of mouse VE-Cadherin by western blot. Samples: Whole cell lysate (2 µg) from NIH 3T3, CT26, bEnd.3, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-VE-Cadherin recombinant monoclonal antibody (orb1566960) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_033998.2 |
|---|
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Protocol Information
Rabbit anti-VE-Cadherin Recombinant Monoclonal Antibody (orb1566960)
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