Rabbit anti-SMAP1 Antibody

• Catalog Number: orb1520640
• Category: Antibodies
• Description: SMAP1 Antibody
• Target: SMAP1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Whole IgG
• Concentration: 1000 µg/ml
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Immunogen: Between 417 and C-terminus
• UniProt ID: Q8IYB5
• Tested applications: IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 8 - 15 µl/mg lysate
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Storage: 2 - 8°C
• Alternative names: SMAP-1; stromal membrane-associated GTPase-activat
• Note: For research use only
• NCBI: Q8IYB5.2
• Expiration Date: 12 months from date of receipt.

Detection of human SMAP1 by western blot. Samples: Nuclear extract (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SMAP1 antibody (orb1520640) used for WB at 0.4 mg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human SMAP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SMAP1 antibody (orb1520640) used for IP at 6 µg per reaction.