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Catalog Number | orb1527770 |
---|---|
Category | Antibodies |
Description | RanBP2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IP, WB |
Reactivity | Human, Mouse |
Immunogen | Between 1050 and 1100 |
Antibody Type | Primary Antibody |
Concentration | 200 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | RanBP2 |
UniProt ID | P49792 |
NCBI | NP_006258.3 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | nuclear pore complex protein Nup358; TRP1; transfo Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human and mouse RanBP2 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RanBP2 antibody orb1527770 used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human RanBP2 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RanBP2 antibody orb1527770 used for IP at 6 µg per reaction.