Rabbit anti-RABGEF1 Antibody

SKU: orb1525754

Description

RABGEF1 Antibody

Images & Validation

• Tested Applications: IHC, IP
• Dilution range: WB - Not recommended; IP - 2 - 5 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 275 and 325
• Target: RABGEF1
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Disclaimer: For research use only

Alternative Names

RAB guanine nucleotide exchange factor (GEF) 1; rab5 GDP/GTP exchange factor; rabaptin-5-associated exchange factor for Rab5; RABEX5; rabex-5; RAP1

Detection of human RABGEF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RABGEF1 antibody orb1525754 used for IP at 3 µg per reaction.

Detection of human RABGEF1 by immunohistochemistry.Sample: FFPE section of human lung cancer. Antibody: Affinity purified rabbit anti-RABGEF1 (Cat. No. orb1525754) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

RABGEF1

UniProt

Q9UJ41

RefSeq:BAC87138.1