Rabbit anti-PSMD4 Antibody

SKU: orb1524052

Description

PSMD4 Antibody

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 327 and C-terminus
• Target: PSMD4

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Disclaimer: For research use only

Alternative Names

multiubiquitin chain-binding protein; S5a/antisecretory factor protein; S5A; Rpn10; proteasome (prosome, macropain) 26S subunit, non-ATPase, 4; MCB1; ASF; angiocidin; 26S proteasome non-ATPase regulatory subunit 4; AF-1; AF; 26S proteasome regulatory subunit S5A; 26S proteasome regulatory subunit RPN10; antisecretory factor 1; pUB-R5

Detection of human PSMD4 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 µg) , HeLa (H; 50 µg) , and Jurkat (J; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PSMD4 antibody orb1524052 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human PSMD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-PSMD4 antibody orb1524052 used for IP at 6 µg/mg lysate.

Detection of human PSMD4 by immunohistochemistry. Samples: FFPE sections of human lung carcinoma. Antibody: Affinity purified rabbit anti-PSMD4 used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

PSMD4

UniProt

P55036

RefSeq (Protein):NP_002801.1