Rabbit anti-Phospho CDK1 (Y15) Recombinant Monoclonal Antibody

• Catalog Number: orb1519743
• Category: Antibodies
• Description: CDK1, Phospho (Y15) Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: BLR101H
• Tested applications: IP, WB
• Reactivity: Human
• Immunogen: Surrounding tyrosine 15
• Antibody Type: Primary Antibody
• Concentration: 40 µg/ml
• Dilution range: WB - 1:1,000; IP - 20 µl/1 mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: CDK1, Phospho (Y15)
• UniProt ID: P06493
• NCBI: NP_001777.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: cyclin-dependent kinase 1; CDC2; p34 protein kinas
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Phospho CDK1 (Y15) by western blot. Samples: Whole cell lysate (50 µg) from HeLa cells treated (+) with Hydroxyurea or mock treated (-) prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho CDK1 (Y15) recombinant monoclonal antibody (orb1519743) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower panel shows WB for total CDK1 using rabbit anti-CDK1 recombinant monoclonal.