Rabbit anti-Phospho CDK1 (Y15) Recombinant Monoclonal Antibody

SKU: orb1519743

Description

CDK1, Phospho (Y15) Antibody

Images & Validation

• Tested Applications: IP, WB
• Dilution range: WB - 1:1,000; IP - 20 µl/1 mg lysate
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Clone No.: BLR101H
• Immunogen: Surrounding tyrosine 15
• Target: CDK1, Phospho (Y15)

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 40 µg/ml
• Disclaimer: For research use only

Alternative Names

cyclin-dependent kinase 1; CDC2; p34 protein kinase; cell division protein kinase 1; cell division cycle 2, G1 to S and G2 to M; cell division control protein 2 homolog; cell cycle controller CDC2; CDK1; CDC28A; P34CDC2

Detection of human Phospho CDK1 (Y15) by western blot. Samples: Whole cell lysate (50 µg) from HeLa cells treated (+) with Hydroxyurea or mock treated (-) prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho CDK1 (Y15) recombinant monoclonal antibody (orb1519743) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower panel shows WB for total CDK1 using rabbit anti-CDK1 recombinant monoclonal.

Quick Database Links

Gene Symbol

CDK1, Phospho (Y15)

UniProt

P06493

RefSeq (Protein):NP_001777.1