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Rabbit anti-Phospho BCKDHA (S292) Recombinant Monoclonal Antibody

Catalog Number: orb1519847

DispatchUsually dispatched within 5-10 working days
$ 250.00
Catalog Numberorb1519847
CategoryAntibodies
DescriptionBCKDHA, Phospho (S292) Antibody
Species/HostRabbit
ClonalityRecombinant
Clone NumberBLR060F
Tested applicationsSW-Size, WB
ReactivityHuman, Mouse
ImmunogenSurrounding serine 292
Antibody TypePrimary Antibody
Concentration40 µg/ml
Dilution rangeWB - 1:1000
Form/AppearanceWhole IgG
ConjugationUnconjugated
TargetBCKDHA, Phospho (S292)
UniProt IDP12694
NCBINP_000700.1
Storage2 - 8°C
Buffer/PreservativesBorate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Alternative namesBCKDH E1-alpha; OVD1A; MSUD1; MSU; branched chain
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NoteFor research use only
Application notesFormat: Whole IgG
Expiration Date12 months from date of receipt.
Rabbit anti-Phospho BCKDHA (S292) Recombinant Monoclonal Antibody

Detection of mouse Phospho BCKDHA (S292) by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, CT26, TCMK-1, BW5147.3, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho BCKDHA (S292) recombinant monoclonal antibody (orb1519847) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Rabbit anti-Phospho BCKDHA (S292) Recombinant Monoclonal Antibody

Detection of human Phospho BCKDHA (S292) by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, GaMG, Hep-G2, HeLa, and LNCaP cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho-BCKDHA-S292 recombinant monoclonal antibody (orb1519847) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Rabbit anti-Phospho BCKDHA (S292) Recombinant Monoclonal Antibody

Detection of human Phospho BCKDHA (S292) by western blot. Samples: Whole cell lysate (50 µg) from HEK293T cells mock treated (-) or treated with phosphatases (+). Antibody: Rabbit anti-Phospho BCKDHA (S292) recombinant monoclonal antibody (orb1519847) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Rabbit anti-Phospho BCKDHA (S292) Recombinant Monoclonal Antibody

Detection of mouse pBCKDHA (S292) by Simple Western™. Samples: Whole cell lysate (0.4 mg/mL) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho BCKDHA (S292) recombinant monoclonal antibody (orb1519847) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram.

Rabbit anti-Phospho BCKDHA (S292) Recombinant Monoclonal Antibody

Detection of human pBCKDHA (S292) by Simple Western™. Samples: Whole cell lysate (0.08 mg/mL) from HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho BCKDHA (S292) recombinant monoclonal antibody (orb1519847) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram.