Rabbit anti-Phospho BCKDHA (S292) Recombinant Monoclonal Antibody

SKU: orb1519847

Description

BCKDHA, Phospho (S292) Antibody

Images & Validation

• Tested Applications: SW-Size, WB
• Dilution range: WB - 1:1000
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Clone No.: BLR060F
• Immunogen: Surrounding serine 292
• Target: BCKDHA, Phospho (S292)
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 40 µg/ml
• Disclaimer: For research use only

Alternative Names

BCKDH E1-alpha; OVD1A; MSUD1; MSU; branched chain keto acid dehydrogenase E1 alpha protein; BCKDE1A; 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial; 2-oxoisovalerate dehydrogenase (lipoamide); branched-chain alpha-keto acid dehydrogenase E1 component alpha chain

Detection of mouse Phospho BCKDHA (S292) by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, CT26, TCMK-1, BW5147.3, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho BCKDHA (S292) recombinant monoclonal antibody (orb1519847) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human Phospho BCKDHA (S292) by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, GaMG, Hep-G2, HeLa, and LNCaP cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho-BCKDHA-S292 recombinant monoclonal antibody (orb1519847) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human Phospho BCKDHA (S292) by western blot. Samples: Whole cell lysate (50 µg) from HEK293T cells mock treated (-) or treated with phosphatases (+). Antibody: Rabbit anti-Phospho BCKDHA (S292) recombinant monoclonal antibody (orb1519847) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse pBCKDHA (S292) by Simple Western™. Samples: Whole cell lysate (0.4 mg/mL) from NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho BCKDHA (S292) recombinant monoclonal antibody (orb1519847) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram.

Detection of human pBCKDHA (S292) by Simple Western™. Samples: Whole cell lysate (0.08 mg/mL) from HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho BCKDHA (S292) recombinant monoclonal antibody (orb1519847) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram.

Quick Database Links

Gene Symbol

BCKDHA, Phospho (S292)

UniProt

P12694

RefSeq (Protein):NP_000700.1