Rabbit anti-PAF49 Antibody

SKU: orb1528665

Description

PAF49 Antibody

Images & Validation

• Tested Applications: ICC, IHC, IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.; ICC-IF - 1:50 - 1:250. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 425 and 475
• Target: PAF49

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Disclaimer: For research use only

Alternative Names

CD3e molecule, epsilon associated protein; RNA polymerase I-associated factor PAF49; PAF49; DNA-directed RNA polymerase I subunit RPA34; CD3E-associated protein; CAST; ASE-1; ASE1; antisense to ERCC-1 protein; A34.5; CD3e antigen, epsilon polypeptide associated protein; CD3-epsilon-associated protein

Detection of human PAF49 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-PAF49 used at a dilution of 1:200 (1µg/ml).

Detection of human PAF49 by WB and IP.Samples: HeLa cells. Antibodies: Affinity purified rabbit anti-PAF49 antibody orb1528665 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate.

Detection of human PAF49 by ICC. Sample: HeLa cells. Antibody: Affinity purified rabbit anti-PAF49 used at a dilution of 1:100. Detection: Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody.

Quick Database Links

Gene Symbol

PAF49

UniProt

O15446

RefSeq (Protein):NP_036231.1