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Catalog Number | orb1530312 |
---|---|
Category | Antibodies |
Description | p300 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ChIP, IHC, IP, WB |
Reactivity | Human |
Immunogen | Between 950 and 1000 |
Antibody Type | Primary Antibody |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:10,000 - 1:25,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | p300 |
UniProt ID | Q09472 |
NCBI | NP_001420.2 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | p300; RSTS2; p300 HAT; KAT3B; histone crotonyltran Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human p300 by western blot.Samples: Whole cell lysate from HEK293T, Jurkat, and HeLa cells. Antibody: Affinity purified rabbit anti-p300 antibody orb1530312 at 0.04 µg/ml.
Detection of human p300 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-p300 used at 1:1, 000 (1µg/ml).
Detection of human p300 by western blot of immunoprecipitates.Samples: Whole cell lysate from HEK293T cells. Antibodies: Affinity purified rabbit anti-p300 antibody.
ELISA, IF, IHC-P, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |