Rabbit anti-OTULIN Antibody

SKU: orb1520374

Description

OTULIN Antibody

Images & Validation

• Tested Applications: IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 20-40 µl/mg lysate
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 75 and 125
• Target: OTULIN

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Disclaimer: For research use only

Alternative Names

OTU domain-containing deubiquitinase with linear linkage specificity; ubiquitin thioesterase Gumby; GUM; FAM105B; deubiquitinating enzyme otulin; AIPDS; ubiquitin thioesterase otulin; family with sequence similarity 105, member B

Detection of human and mouse OTULIN by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, U2OS, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-OTULIN antibody (orb1520374) used for WB at 0.1 mg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human OTULIN by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-OTULIN antibody (orb1520374) used for IP at 2 µg per reaction.

Quick Database Links

Gene Symbol

OTULIN

UniProt

Q96BN8

RefSeq:Q96BN8.3