Rabbit anti-NFIC Antibody

SKU: orb1525419

Description

NFIC Antibody

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 200 and 250
• Target: NFIC

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Disclaimer: For research use only

Alternative Names

Nuclear factor 1/C; TGGCA-binding protein; Nuclear factor I/C; nuclear factor 1 C-type; NFI-C; NF-I/C; NFI; NF1-C; CTF5; CTF; CCAAT-box-binding transcription factor; nuclear factor I/C (CCAAT-binding transcription factor); NF-I

Detection of human NFIC by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NFIC antibody orb1525419 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. NFIC was also immunoprecipitated by rabbit anti-NFIC antibody, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).

Detection of human NFIC by immunohistochemistry. Samples: FFPE serial sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-NFIC Cat. No. orb1525419, left image used at a dilution of 1: 200 (1µg/ml) and Cat. No. orb1525418, right image used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB. Counterstain: Hematoxylin (blue).

Detection of mouse NFIC by immunohistochemistry. Samples: FFPE serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-NFIC Cat. No. orb1525419, left image used at a dilution of 1: 200 (1µg/ml) and Cat. No. orb1525418, right image used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

NFIC

UniProt

P08651

RefSeq:EAW69324.1