Rabbit anti-MNT Antibody

• Catalog Number: orb1524484
• Category: Antibodies
• Description: MNT Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IP, WB
• Reactivity: Human
• Immunogen: Between 1 and 50
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: MNT
• UniProt ID: Q99583
• NCBI: NP_064706.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: ROX; class D basic helix-loop-helix protein 3; MAD
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human MNT by western blot. Samples: Whole cell lysate (10 µg) from HEK293T, HeLa, Hep-G2, LNCaP, and A-549 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MNT antibody (orb1524484) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human MNT by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hep-G2 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MNT antibody (orb1524484) used for IP at 6 µg per reaction. MNT was also immunoprecipitated by a previous lot of this antibody (orb1524484) and another antibody against a different epitope of MNT.