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Catalog Number | orb1523289 |
---|---|
Category | Antibodies |
Description | MAT2A Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IP, WB |
Reactivity | Human, Mouse |
Immunogen | Between 1 and 50 |
Antibody Type | Primary Antibody |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:10,000 - 1:25,000; IP - 2-10 µg/mg lysate |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | MAT2A |
UniProt ID | P31153 |
NCBI | NP_005902.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | methionine adenosyltransferase 2; methionine adeno Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of mouse MAT2A by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MAT2A antibody (orb1523289) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Detection of human MAT2A by western blot. Samples: Whole cell lysate (10 µg) from HeLa, HEK293T, Jurkat, Hep-G2, and K-562 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MAT2A antibody (orb1523289) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of human MAT2A by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MAT2A antibody (orb1523289) used for IP at 6 µg per reaction. MAT2A was also immunoprecipitated by a previous lot of this antibody (orb1523289) and a second antibody against a different epitope of MAT2A.
WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, WB | |
Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |