Rabbit anti-LYRIC/AEG1 Recombinant Monoclonal Antibody

• Catalog Number: orb1710822
• Category: Antibodies
• Description: LYRIC/AEG1 Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: BLR258L
• Tested applications: FC, ICC, IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 475 and 525
• Antibody Type: Primary Antibody
• Concentration: 250 µg/ml
• Dilution range: WB: 1:1,000, IP: 20 µl/1 mg lysate, IHC: 1:50 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections., ICC: 1:50 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: LYRIC/AEG1
• UniProt ID: Q86UE4
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: astrocyte elevated gene-1 protein; protein LYRIC;
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse LYRIC/AEG1 by western blot. Samples: Whole cell lysate (25 µg) from NIH 3T3, ND7/23, CT26, CH27, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-LYRIC/AEG1 recombinant monoclonal antibody (orb1710822) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human LYRIC/AEG1 by western blot. Samples: Whole cell lysate (10 µg) from RKO, LNCaP, A-549, HeLa, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-LYRIC/AEG1 recombinant monoclonal antibody (orb1710822) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human LYRIC/AEG1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Rabbit anti-LYRIC/AEG1 recombinant monoclonal antibody (orb1710822). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human LYRIC/AEG1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-LYRIC/AEG1 recombinant monoclonal antibody (orb1710822). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human LYRIC/AEG1 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-LYRIC/AEG1 recombinant monoclonal antibody (orb1710822) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of mouse LYRIC/AEG1 (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-LYRIC/AEG1 recombinant monoclonal antibody (orb1710822) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human LYRIC/AEG1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from RKO cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-LYRIC/AEG1 recombinant monoclonal antibody (orb1710822) used for IP at 20 µl/mg lysate.

Detection of human LYRIC/AEG1 by immunocytochemistry. Sample: FFPE section of human RKO cells. Antibody: Rabbit anti-LYRIC/AEG1 recombinant monoclonal antibody (orb1710822). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse LYRIC/AEG1 by immunocytochemistry. Sample: FFPE section of mouse CT26 cells. Antibody: Rabbit anti-LYRIC/AEG1 recombinant monoclonal antibody (orb1710822). Secondary: HRP-conjugated goat anti-rabbit IgG.