Rabbit anti-KLF5 Recombinant Monoclonal Antibody

SKU: orb1733252

Description

KLF5 Antibody

Images & Validation

• Tested Applications: FC, ICC, IHC, IP, WB
• Dilution range: WB: 1:1000, IP: 6 µl/mg lysate, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Clone No.: BLR243L
• Immunogen: Between 325 and 375
• Target: KLF5
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Concentration: 1000 µg/ml
• Disclaimer: For research use only

Alternative Names

GC-box-binding protein 2; Kruppel-like factor 5 (intestinal); Krueppel-like factor 5; Klf5C isoform; intestinal-enriched kruppel-like factor; intestinal-enriched krueppel-like factor; intestinal Kruppel-like factor; IKLF; epididymis secretory sperm binding protein; colon kruppel-like factor; colon krueppel-like factor; CKLF; BTE-binding protein 2; BTEB2; Basic transcription element-binding protein 2; basic transcription element binding protein 2; transcription factor BTEB2; GC box binding protein 2

Detection of mouse KLF5 by western blot. Samples: Whole cell lysate (25 µg) from CT26, WEHI-231, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733252) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human KLF5 by western blot. Samples: Whole cell lysate (25 µg) from HeLa, SW620, MCF-7, and KM12 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733252) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse KLF5 by immunohistochemistry. Sample: FFPE section of mouse gut. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733252). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human KLF5 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733252). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human KLF5 (shaded) in HT-29 cells by flow cytometry. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733252) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of mouse KLF5 (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733252) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human KLF5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from KM12 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733252) used for IP at 6 µl/mg lysate.

Detection of human KLF5 by immunocytochemistry. Sample: FFPE section of HT-29 cells. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733252). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse KLF5 by immunocytochemistry. Sample: FFPE section of CT26 cells. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733252). Secondary: HRP-conjugated goat anti-rabbit IgG.

Quick Database Links

Gene Symbol

KLF5

UniProt

Q13887