Rabbit anti-JMJD6 Recombinant Monoclonal Antibody

• Catalog Number: orb1710830
• Category: Antibodies
• Description: JMJD6 Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: BLR254L
• Tested applications: FC, ICC, IHC, WB
• Reactivity: Human, Mouse
• Immunogen: Between 125 and 175
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB: 1:1,000, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections., ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: JMJD6
• UniProt ID: Q6NYC1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Alternative names: peptide-lysine 5-dioxygenase JMJD6; PTDSR1; PTDSR;
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse JMJD6 by western blot. Samples: Whole cell lysate (10 µg) from BW5147.3, NIH 3T3, CH27, ND7/23, and CT26 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-JMJD6 recombinant monoclonal antibody (orb1710830) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human JMJD6 by western blot. Samples: Whole cell lysate (10 µg) from HeLa, HEK293T, Hep-G2, RKO, and OVCAR-8 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-JMJD6 recombinant monoclonal antibody (orb1710830) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human JMJD6 by immunohistochemistry. Sample: FFPE section of human testis. Antibody: Rabbit anti-JMJD6 recombinant monoclonal antibody (orb1710830). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse JMJD6 by immunohistochemistry. Sample: FFPE section of mouse testis. Antibody: Rabbit anti-JMJD6 recombinant monoclonal antibody (orb1710830). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse JMJD6 (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-JMJD6 recombinant monoclonal antibody (orb1710830) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human JMJD6 (shaded) in A549 cells by flow cytometry. Antibody: Rabbit anti-JMJD6 recombinant monoclonal antibody (orb1710830) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human JMJD6 by immunocytochemistry. Sample: FFPE section of human A549 cells. Antibody: Rabbit anti-JMJD6 recombinant monoclonal antibody (orb1710830). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse JMJD6 by immunocytochemistry. Sample: FFPE section of mouse ND7/23 cells. Antibody: Rabbit anti-JMJD6 recombinant monoclonal antibody (orb1710830). Secondary: HRP-conjugated goat anti-rabbit IgG.