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Rabbit anti-JDP2 Recombinant Monoclonal Antibody
Description
Images & Validation
−| Tested Applications | FC, ICC, IHC, WB |
|---|---|
| Dilution range | WB: 1:1000, IHC: 1:40 to 1:100. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections, ICC: 1:40 to 1:100. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE cell sections |
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Recombinant |
| Clone No. | BLR246L |
| Immunogen | Between 50 and 100 |
| Target | JDP2 |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Whole IgG |
| Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
| Concentration | 400 µg/ml |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Rabbit anti-JDP2 Recombinant Monoclonal Antibody [orb1733245]
FC, ICC, IHC, WB
Human, Mouse
Rabbit
Recombinant
Unconjugated
100 μlRabbit anti-JDP2 Recombinant Monoclonal Antibody [orb1733244]
FC, ICC, IHC, WB
Human, Mouse
Rabbit
Recombinant
Unconjugated
100 μg

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Detection of mouse JDP2 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3 and RenCa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733243) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human JDP2 by western blot. Samples: Whole cell lysate (25 µg) from MCF-7, HeLa, HaCaT, and DU145 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733243) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human JDP2 by immunohistochemistry. Sample: FFPE section of human spleen. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733243). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human JDP2 by immunohistochemistry. Sample: FFPE section of human skin carcinoma. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733243). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse JDP2 by immunohistochemistry. Sample: FFPE section of mouse RenCa tumor. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733243). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human JDP2 (shaded) in FaDu cells by flow cytometry. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733243) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of mouse JDP2 (shaded) in CTLL-2 cells by flow cytometry. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733243) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of mouse JDP2 by immunocytochemistry. Sample: FFPE section of mouse C2C12 cells. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733243). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human JDP2 by immunocytochemistry. Sample: FFPE section of human FaDu cells. Antibody: Rabbit anti-JDP2 recombinant monoclonal antibody (orb1733243). Secondary: HRP-conjugated goat anti-rabbit IgG.
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Rabbit anti-JDP2 Recombinant Monoclonal Antibody (orb1733243)
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