Rabbit anti-Interferon alpha/ beta receptor 1 Recombinant Monoclonal Antibody

• Catalog Number: orb1710814
• Category: Antibodies
• Description: Interferon alpha/beta receptor 1 Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: BLR265L
• Tested applications: FC, IHC, IP, WB
• Reactivity: Human
• Immunogen: Residues 27-436 (ECD)
• Antibody Type: Primary Antibody
• Concentration: 400 µg/ml
• Dilution range: WB: 1:1,000, IP: 15 µl/1 mg lysate, IHC: 1:1000 to 1:4000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Interferon alpha/beta receptor 1
• UniProt ID: P17181
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: cytokine receptor family 2 member 1; IFN-R-1; type
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Interferon alpha/beta receptor 1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, RPMI-8226, Jurkat, and My-La CD4+ cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Interferon alpha/beta receptor 1 recombinant monoclonal antibody (orb1710814) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human Interferon alpha/beta receptor 1 by immunohistochemistry. Sample: FFPE section of human appendix. Antibody: Rabbit anti-Interferon alpha/beta receptor 1 recombinant monoclonal antibody (orb1710814). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human Interferon alpha/beta receptor 1 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-Interferon alpha/beta receptor 1 recombinant monoclonal antibody or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human Interferon alpha/beta receptor 1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-Interferon alpha/beta receptor 1 recombinant monoclonal antibody (orb1710814) used for IP at 15 µl/mg lysate.