Rabbit anti-HERC1 Antibody

SKU: orb1527581

Description

HERC1 Antibody

Images & Validation

• Tested Applications: IP, WB
• Dilution range: WB - 1:2000 - 1:10,000; IP - 2 - 10 µg/mg lysate
• Reactivity: Human
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 2400 and 2450
• Target: HERC1
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Disclaimer: For research use only

Alternative Names

HECT domain and RCC1-like domain-containing protein 1; probable E3 ubiquitin-protein ligase HERC1; p619; p532; HECT-type E3 ubiquitin transferase HERC1; hect (homologous to the E6-AP (UBE3A) carboxyl terminus) domain and RCC1 (CHC1)-like domain (RLD) 1; guanine nucleotide exchange factor p532; MDFPMR

Detection of human HERC1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HERC1 antibody orb1527581 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human HERC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa, cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HERC1 antibody orb1527581 used for IP at 3 µg per reaction.

Quick Database Links

Gene Symbol

HERC1

UniProt

Q15751

RefSeq (Protein):NP_003913.3