Rabbit anti-eIF6 Antibody

SKU: orb1525579

Description

eIF6 Antibody

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 150 and 200
• Target: eIF6

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Disclaimer: For research use only

Alternative Names

eukaryotic translation initiation factor 3A; p27(BBP); p27 beta-4 integrin-binding protein; p27BBP; eukaryotic translation initiation factor 6; EIF3A; CAB; B4 integrin interactor; B(2)GCN homolog; b(2)gcn; ITGB4BP; eIF-6

Detection of human and mouse eIF6 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-eIF6 antibody orb1525579 used for WB at 0.1 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. eIF6 was also immunoprecipitated by rabbit anti-eIF6 antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).

Detection of mouse eIF6 by immunohistochemistry. Samples: FFPE serial sections of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-eIF6 used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of human eIF6 by immunohistochemistry. Samples: FFPE serial sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-eIF6 used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB. Counterstain: Hematoxylin (blue).

Quick Database Links

Gene Symbol

eIF6

UniProt

P56537

RefSeq (Protein):NP_852133.1