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Catalog Number | orb1529890 |
---|---|
Category | Antibodies |
Description | DHX8 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human, Mouse |
Immunogen | Between 325 and 375 |
Antibody Type | Primary Antibody |
Concentration | 200 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | DHX8 |
UniProt ID | Q14562 |
NCBI | NP_004932.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | DEAH (Asp-Glu-Ala-His) box polypeptide 8; PRPF22; Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human DHX8 by western blot.Samples: Whole cell lysate from HeLa, HEK293T, and Jurkat cells. Antibody: Affinity purified rabbit anti-DHX8 antibody orb1529890 at 0.1 µg/ml.
Detection of human DHX8 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody:Affinity purified rabbit anti-DHX8 antibody used at 1:1000 (0.2 µg/ml). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of mouse DHX8 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody:Affinity purified rabbit anti-DHX8 antibody used at 1:1000 (0.2 µg/ml). Secondary: HRP-conjugated goat anti-rabbit IgG.
Detection of human DHX8 by western blot of immunoprecipitates.Samples: Whole cell lysate from HeLa cells. Antibodies: Affinity purified rabbit anti-DHX8 antibody.