Rabbit anti-CKAP4 Antibody

SKU: orb1526949

Description

CKAP4 Antibody

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 3 - 5 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 500 and 550
• Target: CKAP4
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Disclaimer: For research use only

Alternative Names

ERGIC-63; transmembrane protein (63kD), endoplasmic reticulum/Golgi intermediate compartment; type-II transmembrane protein p63; p63; CLIMP-63; 63 kDa membrane protein; 63-kDa cytoskeleton-linking membrane protein; cytoskeleton-associated protein 4

Detection of human and mouse CKAP4 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) and mouse NIH 3T3 (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-CKAP4 antibody orb1526949 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. CKAP4 was also immunoprecipitated by rabbit anti-CKAP4 antibodies, which recognize other epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 1 second (B).

Detection of human CKAP4 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-CKAP4 (Cat. No. orb1526949) used at a dilution of 1: 200 (1µg/ml). Detection: DAB

Quick Database Links

Gene Symbol

CKAP4

UniProt

Q07065

RefSeq (Protein):NP_006816.2