Rabbit anti-CHAMP1 Antibody

• Catalog Number: orb1523405
• Category: Antibodies
• Description: CHAMP1 Antibody
• Target: CHAMP1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Whole IgG
• Concentration: 1000 µg/ml
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Immunogen: Between 762 and C-terminus
• UniProt ID: Q96JM3
• Tested applications: ICC, IHC, IP, WB
• Dilution range: WB - 1:1,000 - 1:5,000; IP - 2 - 10 µg/mg lysate
• Application notes: Format: Whole IgG
• Antibody Type: Primary Antibody
• Storage: 2 - 8°C
• Alternative names: MRD40; zinc finger protein 828; ZNF828; CHAMP; C13
• Note: For research use only
• NCBI: NP_115812.1

Detection of human and mouse CHAMP1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-CHAMP1 antibody orb1523405 used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human CHAMP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-CHAMP1 antibody orb1523405 used for IP at 6 µg/mg lysate.

Detection of human CHAMP1 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CHAMP1 (Cat. No. orb1523405) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB