Rabbit anti-CD86 Recombinant Monoclonal Antibody

• Catalog Number: orb1710828
• Category: Antibodies
• Description: CD86 Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: BLR255L
• Tested applications: FC, ICC, IP, WB
• Reactivity: Mouse
• Immunogen: Residues 24-245 (ECD)
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB: 1:1,000, IP: 6 µl/1 mg lysate, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: CD86
• UniProt ID: P42082
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Alternative names: Early T-cell costimulatory molecule 1; B7.2; T-lym
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse CD86 by western blot. Samples: Whole cell lysate (10 µg) from A20, CH27, WEHI-231, J774A1, and Ba/F3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD86 recombinant monoclonal antibody (orb1710828) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse CD86 (shaded) in CH27 cells by flow cytometry. Antibody: Rabbit anti-CD86 recombinant monoclonal antibody (orb1710828) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of mouse CD86 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from CH27 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD86 recombinant monoclonal antibody (orb1710828) used for IP at 6 µl/mg lysate.

Detection of mouse CD86 by immunocytochemistry. Sample: FFPE section of mouse CH27 cells. Antibody: Rabbit anti-CD86 recombinant monoclonal antibody (orb1710828). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse CD86 by immunocytochemistry. Sample: FFPE section of mouse J774A1 cells. Antibody: Rabbit anti-CD86 recombinant monoclonal antibody (orb1710828). Secondary: HRP-conjugated goat anti-rabbit IgG.