Rabbit Bovine beta lactoglobulin Antibody

• Catalog Number: orb21203
• Category: Antibodies
• Description: Precipitating polyclonal rabbit antiserum to bovine beta-lactoglobulin. Beta-lactoglobulin is the dominant protein in bovine milk, synthesized in mammary gland tissue. It is a glycoprotein present mainly as a dimer at intermediate concentrations near the isoelectric point. The molecular weight of the monomeric subunit is near 17,000. It is very sensitive to denaturation. Any treatment of native milk and also prolonged storage in the deepfreeze will lead first to polymerization (octamers and higher polymers) aggregation and finally to denaturation. Three genetic variants A, B and C have been identified. They differ very little in the amino acid constellation an their behavior under pH and temperature various conditions. No immunological differences was found between the variants. Beta-lactoglobulin can be purified in crystallized form. Allergic reactions of infants to bovine milk proteins are well known. The actual process by which such reactions develop and their impact on the infant's health have not been unambiguously established. Circulating antibodies to beta-lactoglobulin have been demonstrated in sera of some infants. Freund's complete adjuvant is used in the first step of the immunization procedure.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Bovine
• Form/Appearance: Delipidated, heat inactivated, lyophilized, stable whole antiserum No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those in pooled rabbit serum. No foreign proteins added.
• RRID: AB_10753903
• Antibody Type: Primary Antibody
• Source: Beta-lactoglobulin is the dominant protein in bovine milk, synthesized in mammary gland tissue. It is a glycoprotein present mainly as a dimer at intermediate concentrations near the isoelectric point. The molecular weight of the monomeric subunit is near 17,000. It is very sensitive to denaturation. Any treatment of native milk and also prolonged storage in the deepfreeze will lead first to polymerization (octamers and higher polymers) aggregation and finally to denaturation. Three genetic variants A, B and C have been identified. They differ very little in the amino acid constellation an their behavior under pH and temperature various conditions. No immunological differences was found between the variants. Beta-lactoglobulin can be purified in crystallized form. Allergic reactions of infants to bovine milk proteins are well known. The actual process by which such reactions develop and their impact on the infant’s health have not been unambiguously established. Circulating antibodies to beta-lactoglobulin have been demonstrated in sera of some infants. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only

Immunoelectrophoresis shows beta Lactoglobulin antibody containing antiserum reaction with cow milk and purified beta lactoglobulin but not with serum

Ouchterlony immunodiffusion showing beta Lactoglobulin antibody containing antiserum strong reaction with an antigen in bovine milk but not with serum

Immunoprecipitation of Beta Lactoglobulin antibody containing antiserum with bovine milk