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Catalog Number | orb1527129 |
---|---|
Category | Antibodies |
Description | BOP1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human, Mouse |
Immunogen | Between 375 and 425 |
Antibody Type | Primary Antibody |
Concentration | 200 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IHC - 1:200 to 1:1000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | BOP1 |
UniProt ID | Q14137 |
NCBI | NP_056016.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | Block of proliferation 1 protein; ribosome biogene Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human BOP1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, K-562, and RPMI-8226 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BOP1 antibody (orb1527129) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Detection of human BOP1 by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BOP1 (orb1527129) used at a dilution of 1: 200 (1µg/ml). Detection: DAB