Rabbit anti-BCKDHA, Phospho (S292) Antibody

SKU: orb1522550

Description

BCKDHA, Phospho (S292) Antibody

Images & Validation

• Tested Applications: WB
• Dilution range: WB - 1:10,000 - 1:25,000
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Surrounding serine 292
• Target: BCKDHA, Phospho (S292)
• Conjugation: Unconjugated

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
• Concentration: 1000 µg/ml
• Disclaimer: For research use only

Alternative Names

BCKDH E1-alpha; OVD1A; MSUD1; MSU; branched chain keto acid dehydrogenase E1 alpha protein; BCKDE1A; 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial; 2-oxoisovalerate dehydrogenase (lipoamide); branched-chain alpha-keto acid dehydrogenase E1 component alpha chain

Detection of mouse pBCKDHA (S292) by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-pBCKDHA (S292) antibody (orb1522550) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human pBCKDHA (S292) by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells treated with phosphatase (+) or mock treated (-). Antibody: Affinity purified rabbit anti-pBCKDHA (S292) antibody (orb1522550) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-BCKDHA antibody.

Detection of human pBCKDHA (S292) by western blot. Samples: Whole cell lysate (10 µg) from Hep-G2, HeLa, and LNCaP cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-pBCKDHA (S292) antibody (orb1522550) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Quick Database Links

Gene Symbol

BCKDHA, Phospho (S292)

UniProt

P12694

RefSeq (Protein):NP_000700.1