Procollagen type IIA Rabbit Polyclonal Antibody

• Catalog Number: orb2876
• Category: Antibodies
• Description: Procollagen type IIA Rabbit Polyclonal Antibody
• Target: COL2A1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Gallus, Guinea pig, Human, Porcine, Rabbit, Sheep, Zebrafish
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human Collagen II alpha 1 N-terminal propeptide (1-100/1487aa)
• UniProt ID: P02458
• MW: 17/163 kDa
• Tested applications: ELISA, ICC, IF, IHC-Fr, IHC-P
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Collagen II N-terminal propeptide; Collagen II alp
• Note: For research use only

Paraffin-embedded human bladder carcinoma fixed with 4% paraformaldehyde. Antigen retrieval by boiling with citrate buffer. Blocking buffer is goat serum (37 degrees for 20 min.). procollagen type IIA antibody at 1:200 dilution with overnight incubation a