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POLE3 Antibody
SKU: orb1274436
Featured
Description
Images & Validation
−Item 1 of 4
| Tested Applications | IF, WB |
|---|---|
| Reactivity | Human, Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human POLE3 (NP_059139.3). |
| Target | POLE3 |
| Molecular Weight | Observed: 20-25kDa |
| Purification | Affinity purification |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−POLE3, CHARAC17, CHRAC17, YBL1, p17
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Immunofluorescence analysis of U2OS cells using POLE3 antibody (orb1274436).
Western blot analysis of extracts of various cell lines, using POLE3 antibody (orb1274436) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Western blot analysis of extracts from normal (control) and POLE3 knockout (KO) 293T cells, using POLE3 antibody (orb1274436) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunofluorescence analysis of U2OS cells using POLE3 antibody (orb1274436). Green：gamma H2AX (phospho S139) used for DNA damage marker. Blue: DAPI for nuclear staining. Cells were treated by UV-A laser.
Quick Database Links
Gene Symbol
POLE3
UniProt
UniProt Details
− No UniProt data available
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
POLE3 Antibody (orb1274436)
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