Placental Alkaline Phosphatase Antibody / ALPP

SKU: orb639466

Description

Reacts with a 70kDa membrane-bound isozyme (Regan and Nagao type) of placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease.

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution range: Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (2),Western blot: 1-2ug/ml
• Reactivity: Human
• Application Notes:
  Optimal dilution of the Placental Alkaline Phosphatase antibody should be determined by the researcher.1. No special pretreatment is required for the immunohistochemical staining of formalin-fixed tissues.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b, kappa
• Clone No.: ALP/870
• Immunogen: Recombinant full-length human ALPP protein was used as the immunogen for the Placental Alkaline Phosphatase antibody.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Western blot testing of human JEG-3 cells with Placental Alkaline Phosphatase antibody (clone ALP/870). Predicted molecular weight ~58 kDa but routinely visualized at 60-70 kDa.

SDS-PAGE analysis of purified, BSA-free Placental Alkaline Phosphatase antibody (clone ALP/870) as confirmation of integrity and purity.

IHC: Formalin-fixed, paraffin-embedded human placenta stained with Placental Alkaline Phosphatase antibody (clone ALP/870).

Quick Database Links

UniProt

P05187