PFK-2 car (phospho Ser483) rabbit pAb

SKU: orb764365

Description

Rabbit polyclonal antibody to PFK-2 car (phospho-Ser483)

Images & Validation

• Tested Applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human PFKFB2 around the phosphorylation site of Ser483. AA range:451-500
• Molecular Weight: 58
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-PFKFB2 antibody, anti-6-phosphofructo-2-kinase/fructose-2 antibody, anti-6-bisphosphatase 2 antibody, anti-6PF-2-K/Fru-2 antibody, anti-6-P2ase 2 antibody, anti-PFK/FBPase 2 antibody, anti-6PF-2-K/Fru-2 antibody, anti-6-P2ase heart-type isozyme antibody

Western Blot analysis of various cells using Phospho-PFK-2 car (S483) Polyclonal Antibody diluted at 1:1000.

Western Blot analysis of Hela cells using Phospho-PFK-2 car (S483) Polyclonal Antibody diluted at 1:1000.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PFKFB2 (Phospho-Ser483) Antibody.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using PFKFB2 (Phospho-Ser483) Antibody. The picture on the right is blocked with the phospho peptide.