PDE4B/C/D (phospho Ser133/119/190) rabbit pAb Antibody

SKU: orb769399

Description

Rabbit polyclonal antibody to PDE4B/C/D (phospho-Ser133/119/190)

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human PDE4D around the phosphorylation site of Ser190/53. AA range:156-205
• Molecular Weight: 76
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-PDE4B antibody, anti-DPDE4 antibody, anti-cAMP-specific 3' antibody, anti-5'-cyclic phosphodiesterase 4B antibody, anti-DPDE4 antibody, anti-PDE32 antibody, anti-PDE4C antibody, anti-DPDE1 antibody, anti-cAMP-specific 3' antibody, anti-5'-cyclic phosphodiesterase 4C antibody, anti-DPDE1 antibody, anti-PDE21 antibody, anti-PDE4D antibody, anti-DPDE3 antibody, anti-cAMP-specific 3' antibody, anti-5'-cyclic phos antibody

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PDE4D (Phospho-Ser190/53) Antibody.

Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using PDE4D (Phospho-Ser190/53) Antibody. The picture on the right is blocked with the phospho peptide.

Western blot analysis of lysates from K562 cells treated with H2O2 100uM 30', using PDE4D (Phospho-Ser190/53) Antibody. The lane on the right is blocked with the phospho peptide.