PCNA Antibody

SKU: orb2639046

Description

Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This mAb is excellent for multiple applications.

Images & Validation

• Tested Applications: FACS, IHC-P, WB
• Dilution range: Flow cytometry: 1-2ug/10^6 cells,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the PCNA antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a, kappa
• Clone No.: PCNA/694
• Immunogen: Recombinant full length human protein was used as the immunogen for the PCNA antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Disclaimer: For research use only

Flow cytometry testing of PFA-fixed human HeLa cells with PCNA antibody (clone PCNA/694); Red = isotype control, Blue = PCNA antibody.

IHC: Formalin-fixed, paraffin-embedded human tonsil stained with PCNA antibody (clone PCNA/694).

Western blot analysis of HepG2 cell lysate using PCNA antibody (clone PCNA/694). Predicted molecular weight ~29 kDa, routinely observed at 29~36 kDa.

Quick Database Links

UniProt

P12004