p70 S6 kinase α (phospho Thr421) rabbit pAb

SKU: orb769963

Description

Rabbit polyclonal antibody to p70 S6 kinase alpha (phospho-Thr421)

Images & Validation

• Tested Applications: ELISA, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Synthesized phospho-peptide around the phosphorylation site of human p70 S6 kinase α (phospho Thr421)
• Molecular Weight: 70
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-RPS6KB1 antibody, anti-STK14A antibody, anti-Ribosomal protein S6 kinase beta-1 antibody, anti-S6K-beta-1 antibody, anti-S6K1 antibody, anti-70 kDa ribosomal protein S6 kinase 1 antibody, anti-P70S6K1 antibody, anti-p70-S6K 1 antibody, anti-Ribosomal protein S6 kinase I antibody, anti-Serine/threonine-protein kinase 14A antibody, anti-p70 antibody

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using p70 S6 Kinase (Phospho-Thr421) Antibody.

Immunohistochemistry analysis of paraffin-embedded human breast cancer, using p70 S6 Kinase (Phospho-Thr421) Antibody. The picture on the right is blocked with the p70 S6 Kinase (Phospho-Thr421) peptide.