p35 rabbit pAb

SKU: orb765985

Description

Rabbit polyclonal antibody to p35

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Dilution range: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
• Reactivity: Human, Mouse, Rat

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human CDK5R1. AA range:11-60
• Molecular Weight: 38
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-CDK5R1 antibody, anti-CDK5R antibody, anti-NCK5A antibody, anti-Cyclin-dependent kinase 5 activator 1 antibody, anti-CDK5 activator 1 antibody, anti-Cyclin-dependent kinase 5 regulatory subunit 1 antibody, anti-TPKII regulatory subunit antibody

Immunofluorescence analysis of Hela cell. 1, p35 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Caspase 9 Monoclonal Antibody (3-20) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).

Western Blot analysis of various cells using p35 Polyclonal Antibody diluted at 1:1000.

Western Blot analysis of HELA cells using p35 Polyclonal Antibody diluted at 1:1000.

Immunofluorescence analysis of HeLa cells, using CDK5R1 Antibody. The picture on the right is blocked with the synthesized peptide.